3^rd International Conference on Microbiome, Probiotics & Gut Nutrition December 06-07, 2019 Vancouver, Canada

Biography:

Afroza Parvin is a faculty member of Jahangirnagar University in Bangladesh and now she is in study leave from her profession and pursuing her MSc degree in Biosystems Engineering at University of Manitoba, Canada. She is already holding a BSc amd MSc degree in Biochemistry and Molecular Biology. Afroza has 9 years of teaching and research experiences, mostly doing microbiological research. She has already 11 peer reviewed publications. Afroza grew up in the capital city of Bangladesh and is enthusiastic about the research area of environmental microbiology.

Abstract:

In this present study, both lactose and non-lactose fermenting bacterial isolates were isolated from five different textile industrial canals of Bangladesh in 2016. Total bacterial colony (TBC) was counted for day 1 to day 5 for 10-6 dilution to 10-10 dilution. All the isolates were isolated and selected using 4 differential media, and tested for minimum inhibitory concentration (MIC) of heavy metals, and antibiotics susceptibility estimation. Binary exposure experiment, and plasmid profilings were also done. The colony forming units (CFU) per plate were countable mostly for 10-10 dilution. A total of 50 Shigella, 50 Salmonella, and 100 E.coli (Esherichia coli) like bacterial isolates were selected for this study where the MIC was ≤ 0.6 mM for 100% Shigella and Salmonella like isolates, however, only 3% E. coli like isolates had the same MIC for nickel (Ni). The MIC for chromium (Cr) was ≤ 2.0 mM for 16% Shigella, 20% Salmonella, and 17% E. coli like isolates. Around 60% of both Shigella and Salmonella, but only 20% E.coli like isolates had a MIC of ≤ 1.2 mM for lead (Pb). The most prevalent resistant pattern for azithromycin (AZM) for Shigella and Salmonella like isolates was found 38% and 48%, respectively, however, for E.coli like isolates, the highest pattern (36%) was found for sulfamethoxazole-trimethoprim (SXT). In the binary exposure experiment, antibiotic zone of inhibition was mostly increased in the presence of heavy metals. The highest sized plasmid was found 21 Kb and 14 Kb for lactose and non-lactose fermenting isolates, respectively.

Biography:

Reda M El Badawy has completed her MD at Banha University, Faculty Medicine. She is working at King Saud University and King Khaled University, Saudi Arabia. She has published more than 25 papers in reputed journals.

Abstract:

Background & Objectives: Still there is percentage of HCV patients not responding to Direct Antiviral Agents (DAAS), even the responder HCV patients needed to follow up. Gut flora (Microbiota) include all the microorganisms (its gene called 16sRNA microbiome). Liver can be greatly affected by changes in gut microbiome. This study was done to evaluate the association between gut flora and the response to DAAS in chronic HCV patients.

Methodology: Two groups, group 1(No=15 of HCV responders patients) and group 2(No=15 non responder HCV patients) treated by DAAS according to the treatment protocol of the Egyptian National Committee for Control of Viral Hepatitis (NCCVH). Healthy control subjects (No=15) as third group age and sex matched compared to of HCV patients. All investigations were done plus stool culture & PCR for gene extraction by sequencing according to the manufacturer.

Results: The results show statistically significant difference between the patients (responders and non responders) and control, where p1 comparison between contro and responders, p2 between control and non responders, p3 between responders and non responders. Enterobacter organism in p2, p3 was <0.001&<0.005 respectively. Proteus and Clostridium perfringens in p2 was 0.009, 0.04 respectively .Klebsiella organism in p1, p2 was 0.04 and 0.01 respectively while Streptococci in p2 was 0,007.

Conclusion: Gut microbiome a have a crucial role in HCV patients specially the non responders compared to the control even the responders need to be followed up to adjust the gut flora of them to the normal because this proved to play an important role in micro environmental changes that lead to hepatocellular carcinoma(HCC).

Biography:

Abstract:

Biography:

Professor Dr. A. R. M. Solaiman did PhD from The Queen’s University of Belfast and Postdoctoral research in University of Aberdeen, UK. He served as Visiting Scholar in Rhine-Waal University, Germany. Professor Solaiman is involved in teaching and research for 38 years. He is a senior teacher of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh and also holding the position of Director (International Affairs). He served as Dean, Faculty of Graduate Studies and Director (Research). Professor Solaiman has published 140 research articles in reputed journals. He served as research supervisor for 15 PhD and 27 MS students.

Abstract:

Diazotrophic bacteria are thought to replace part of N fertilizer required by rice plant. To this context an initial experiment was conducted in pot to assess the influence of fifteen diazotrophic Rhizobium isolates viz. BU Ls 2, BU Ls 6, BU Ls 13, BU Ls 14, BU Ls 15, BU Ls 16, BU Ls 17, BU Ls 18, BU Ca 6, BU Ca 7, BU Ca 8, BU Le 9, BU Le 10, BU Ps 1 and BU Ps 2 on growth, yield and nutrient uptake. Among the isolates BU Ls 6 performed best in recording plant height, root length and volume, biomass and grain yields and nutrient uptake both at vegetative and harvesting stages of rice. BU Ls 2 also performed more or less similarly as that of BU Ls 6. A follow up experiment was conducted in field to assess the influence of the isolates in presence and absence of N and P supplied from fertilizers. BU Ls 6 in combination with recommended doses of 50%N +100%P significantly increased all plant parameters including biomass (5.24 t ha-1) and grain (4.916 t ha-1) yields and nutrient uptake. Among the isolates BU Ls 6 was the best diazotrophic bacteria.

Biography:

Noriko Komatsuzaki has studied a germinated brown rice and lactic acid bacteria (LAB) in National Food Research Institute for 10 years (1997-2007). She works in Seitoku University from 2008. Research category is Food microbiology as LAB and yeast. L. paracasei NFRI 7415 isolated from traditional Japanese fermented fish, exhibits high g-aminobutyric acid producing ability (Komatsuzaki et al., 2005). It was found that this strain is used in the development of functional fermented foods. At present study, we investigate whether this strain has an immune stimulation effect and wound healing effect.

Abstract:

We examined the probiotic effect of Lactobacillus paracasei NFRI 7415 isolated from Japanese fish (funa-sushi), evaluating it with in vitro and in vivo assay systems and using Lactobacillus casei for comparison. Bacteria were grown to the stationary phase in MRS medium. The medium was then freeze-dried and used in the experiments. In in vitro assays, the cholesterol adsorption rate of L. casei and L. paracasei were 39.4% and 50.9%, respectively, and bile acid adsorption rates 33.8% and 40.9%. Cholesterol adsorption was higher in L. paracasei than in L. casei, and it was revealed that the survival rate of L. paracasei in the stomach was high. In addition, L. paracasei showed gastric juice resistance and bile tolerance. In order to investigate organic acid in appendix contents, male ICR mice were divided into two feeding groups for 4 weeks: a control (CO) group and a lactic acid bacterium (LB) group. Among organic acids in appendix contents, acetic acid showed the highest value in both groups. In the LB group, lactic acid and iso-butyric acid were also detected, but they were not detected in the CO group. These organic acids are a metabolism product produced in intestinal bacteria, and therefore indicate that L. paracasei had an influence on the intestinal microflora of the LB group. This suggests that L. paracasei NFRI 7415 may have potential as a probiotics product.